Nuclear localization of mouse fibroblast growth factor 2 requires N-terminal and C-terminal sequences.
Détails
Etat: Public
Version: Final published version
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
ID Serval
serval:BIB_E9234B690F68
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Nuclear localization of mouse fibroblast growth factor 2 requires N-terminal and C-terminal sequences.
Périodique
Cellular and Molecular Life Sciences
ISSN
1420-682X
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
60
Numéro
10
Pages
2254-2265
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
In vertebrates, different isoforms of fibroblast growth factor 2 (FGF2) exist, which differ by their N-terminal extension. They show different localization and expression levels and exert distinct biological effects. Nevertheless, genetic inactivation of all FGF2 isoforms in the mouse results in only mild phenotypes. Here, we analyzed mouse FGF2, and show that, as in the human, mouse FGF2 contains CTG-initiated high molecular-weight (HMW) isoforms, which contain a nuclear localization signal, and which mediate localization of this isoform to the nucleus. Using green fluorescent protein-FGF2 fusions, we furthermore observed, that C-terminal deletions disable nuclear localization of the short low-molecular-weight (LMW) 18-kDa isoform. This loss of specific localization is accompanied by a loss in heparin binding. We therefore suggest that, first, localization of mouse FGF2 is comparable to that in other vertebrates and, second, FGF2 contains at least two sequences important for nuclear localization, a nuclear localization sequence at the N terminus which is only contained in the HMW isoform, and another sequence at the C terminus, which is only required for localization of the LMW 18-kDa isoform.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Cell Nucleus, Fibroblast Growth Factor 2, Genes, Reporter, Heparin, Mice, Molecular Sequence Data, Protein Isoforms, Protein Sorting Signals, Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/01/2008 17:06
Dernière modification de la notice
14/02/2022 8:57