Nuclear localization of mouse fibroblast growth factor 2 requires N-terminal and C-terminal sequences.

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Serval ID
serval:BIB_E9234B690F68
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nuclear localization of mouse fibroblast growth factor 2 requires N-terminal and C-terminal sequences.
Journal
Cellular and Molecular Life Sciences
Author(s)
Foletti A., Vuadens F., Beermann F.
ISSN
1420-682X
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
60
Number
10
Pages
2254-2265
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
In vertebrates, different isoforms of fibroblast growth factor 2 (FGF2) exist, which differ by their N-terminal extension. They show different localization and expression levels and exert distinct biological effects. Nevertheless, genetic inactivation of all FGF2 isoforms in the mouse results in only mild phenotypes. Here, we analyzed mouse FGF2, and show that, as in the human, mouse FGF2 contains CTG-initiated high molecular-weight (HMW) isoforms, which contain a nuclear localization signal, and which mediate localization of this isoform to the nucleus. Using green fluorescent protein-FGF2 fusions, we furthermore observed, that C-terminal deletions disable nuclear localization of the short low-molecular-weight (LMW) 18-kDa isoform. This loss of specific localization is accompanied by a loss in heparin binding. We therefore suggest that, first, localization of mouse FGF2 is comparable to that in other vertebrates and, second, FGF2 contains at least two sequences important for nuclear localization, a nuclear localization sequence at the N terminus which is only contained in the HMW isoform, and another sequence at the C terminus, which is only required for localization of the LMW 18-kDa isoform.
Keywords
Amino Acid Sequence, Animals, Base Sequence, Cell Nucleus, Fibroblast Growth Factor 2, Genes, Reporter, Heparin, Mice, Molecular Sequence Data, Protein Isoforms, Protein Sorting Signals, Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Yes
Create date
17/01/2008 17:06
Last modification date
01/10/2019 7:20
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