Exposure to bioaerosols in poultry houses at different stages of fattening: use of real-time PCR for airborne bacterial quantification

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Ressource 1Télécharger: BIB_E80A6A0D1CA3.P001.pdf (322.84 [Ko])
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_E80A6A0D1CA3
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Institution
Titre
Exposure to bioaerosols in poultry houses at different stages of fattening: use of real-time PCR for airborne bacterial quantification
Titre de la conférence
Regenerative medicine, CHUV Research Day, January 17, 2008
Auteur⸱e⸱s
Oppliger Anne, Charrière Nicole, Droz Pierre-Olivier, Rinsoz Thomas
Editeur
Université de Lausanne, Faculté de biologie et de médecine
Adresse
Lausanne
Statut éditorial
Publié
Date de publication
2008
Pages
ENA-1, 23
Langue
anglais
Notes
Type de document : Abstracts SAPHIRID:67685
Résumé
Previous studies have demonstrated that poultry-house workers are exposed to very high levels of organic dust and consequently have an increased prevalence of adverse respiratory symptoms. However, the influence of the age of broilers, on bioaerosol concentrations has not been investigated. To evaluate the evolution of bioaerosol concentration during the fattening period, bioaerosol parameters (inhalable dust, endotoxin and bacteria) were measured in 12 poultry confinement buildings in Switzerland, at 3 different stages of the birds' growth; Samples of air taken from within the breathing zones of individual poultry-house employees as they caught the chickens ready to be transported for slaughter, were also analysed. Quantitative PCR (Q-PCR) was used to assess the quantity of total airborne bacteria and total airborne Staphylococcus species. Bioaerosol levels increased significantly during the fattening period of the chickens. During the task of catching mature birds, the mean inhalable dust concentration for a worker was 31 ± 4.7 mg/m3, and endotoxin concentration was 11'080 ± 3436 UE/m3 air, more than ten-fold higher than the Swiss occupational recommended value (1000 UE/m3). The mean exposure level of bird catchers to total bacteria and Staphylococcus species measured by Q-PCR is also very high, respectively reaching values of 72 (± 11) x107 cells/m3 air and 70 (± 16) x106/m3 air. It was concluded that in the absence of wearing protective breathing apparatus, chicken catchers in Switzerland risk exposure beyond recommended limits for all measured bioaerosol parameters. Moreover, the use of Q-PCR to estimate total and specific numbers of airborne bacteria is a promising tool for evaluating any modifications intended to improve the safety of current working practices.
Mots-clé
Aerosols , Air Pollutants, Occupational , Endotoxins , Environmental Monitoring , Industry , Occupational Exposure
Création de la notice
18/03/2009 12:16
Dernière modification de la notice
20/08/2019 16:10
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