Small GTPase CDC-42 promotes apoptotic cell corpse clearance in response to PAT-2 and CED-1 in C. elegans.
Détails
ID Serval
serval:BIB_E4E0A2FA2146
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Small GTPase CDC-42 promotes apoptotic cell corpse clearance in response to PAT-2 and CED-1 in C. elegans.
Périodique
Cell death and differentiation
ISSN
1476-5403 (Electronic)
ISSN-L
1350-9047
Statut éditorial
Publié
Date de publication
06/2014
Peer-reviewed
Oui
Volume
21
Numéro
6
Pages
845-853
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
The rapid clearance of dying cells is important for the well-being of multicellular organisms. In C. elegans, cell corpse removal is mainly mediated by three parallel engulfment signaling cascades. These pathways include two small GTPases, MIG-2/RhoG and CED-10/Rac1. Here we present the identification and characterization of CDC-42 as a third GTPase involved in the regulation of cell corpse clearance. Genetic analyses performed by both loss of cdc-42 function and cdc-42 overexpression place cdc-42 in parallel to the ced-2/5/12 signaling module, in parallel to or upstream of the ced-10 module, and downstream of the ced-1/6/7 module. CDC-42 accumulates in engulfing cells at membranes surrounding apoptotic corpses. The formation of such halos depends on the integrins PAT-2/PAT-3, UNC-112 and the GEF protein UIG-1, but not on the canonical ced-1/6/7 or ced-2/5/12 signaling modules. Together, our results suggest that the small GTPase CDC-42 regulates apoptotic cell engulfment possibly upstream of the canonical Rac GTPase CED-10, by polarizing the engulfing cell toward the apoptotic corpse in response to integrin signaling and ced-1/6/7 signaling in C. elegans.
Mots-clé
Animals, Apoptosis/genetics, Caenorhabditis elegans/genetics, Caenorhabditis elegans/growth & development, Caenorhabditis elegans Proteins/genetics, Caenorhabditis elegans Proteins/metabolism, Cell Adhesion Molecules/metabolism, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, GTP-Binding Proteins/genetics, GTP-Binding Proteins/metabolism, Gene Expression Regulation, Developmental, Guanine Nucleotide Exchange Factors/metabolism, Integrin beta Chains/genetics, Membrane Proteins/genetics, Membrane Proteins/metabolism, Monomeric GTP-Binding Proteins/genetics, Monomeric GTP-Binding Proteins/metabolism, Signal Transduction/genetics, rac GTP-Binding Proteins/metabolism
Pubmed
Web of science
Site de l'éditeur
Open Access
Oui
Création de la notice
08/12/2023 10:45
Dernière modification de la notice
09/12/2023 8:03