Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis.

Détails

ID Serval
serval:BIB_E4D060C2ACF2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis.
Périodique
Electrophoresis
Auteur(s)
Busnel J.M., Lion N., Girault H.H.
ISSN
0173-0835 (Print)
ISSN-L
0173-0835
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
29
Numéro
7
Pages
1565-1572
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Electrokinetic supercharging has been integrated in CZE for the development of a highly sensitive methodology for protein tryptic digest analysis. A careful choice of the experimental conditions led to sensitivity enhancement factors between 1000 and 10,000 whilst maintaining a satisfactory resolution. Peptides in the low nanomolar concentration range have been detected despite the use of the poorly sensitive UV absorbance detection mode. The buffer system used in this study is fully suitable for coupling CE to MS.
Mots-clé
Electrophoresis, Capillary/methods, Peptides/chemistry, Sensitivity and Specificity, Spectrophotometry, Ultraviolet
Pubmed
Web of science
Création de la notice
09/11/2014 22:43
Dernière modification de la notice
20/08/2019 16:08
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