Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis.

Details

Serval ID
serval:BIB_E4D060C2ACF2
Type
Article: article from journal or magazin.
Collection
Publications
Title
Electrokinetic supercharging for highly efficient peptide preconcentration in capillary zone electrophoresis.
Journal
Electrophoresis
Author(s)
Busnel J.M., Lion N., Girault H.H.
ISSN
0173-0835 (Print)
ISSN-L
0173-0835
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
29
Number
7
Pages
1565-1572
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Electrokinetic supercharging has been integrated in CZE for the development of a highly sensitive methodology for protein tryptic digest analysis. A careful choice of the experimental conditions led to sensitivity enhancement factors between 1000 and 10,000 whilst maintaining a satisfactory resolution. Peptides in the low nanomolar concentration range have been detected despite the use of the poorly sensitive UV absorbance detection mode. The buffer system used in this study is fully suitable for coupling CE to MS.
Keywords
Electrophoresis, Capillary/methods, Peptides/chemistry, Sensitivity and Specificity, Spectrophotometry, Ultraviolet
Pubmed
Web of science
Create date
09/11/2014 22:43
Last modification date
20/08/2019 16:08
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