Short-term culture of spleen lymphocytes for chromosome analysis of small rodents.

Détails

ID Serval
serval:BIB_E46C2F7C1B44
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Short-term culture of spleen lymphocytes for chromosome analysis of small rodents.
Périodique
Genetica
Auteur(s)
Jotterand Bellomo M., Mühlematter D., Nabholz M.
ISSN
0016-6707
Statut éditorial
Publié
Date de publication
1990
Volume
83
Numéro
1
Pages
51-60
Langue
anglais
Résumé
In order to improve the methods for the cytogenetic analysis of small rodents with regard to metaphase number, quality of chromosome resolution and duration of cultivation, a short-term culture technique from spleen cells, based on the capacity of Concanavalin A supernatant to stimulate the proliferation of normal T lymphocytes, was developed. Protocols of culture and chromosome preparation are described in detail. This technique not only makes it possible to obtain a high number of cells in mitosis from the spleen of very small rodents in a short time, but also insures metaphases of good quality, due to optimal control of cell cycle and processing thanks to cultivation in small aliquots. Although the method applies mainly to rodents because of species specificity of interleukin 2, it can probably be easily adapted to species from other mammalian orders.
Mots-clé
Animals, Arvicolinae, Chromosome Banding, Chromosomes, Cytogenetics, Female, Karyotyping, Lymphocytes, Mice, Muridae, Rodentia, Spleen
Pubmed
Création de la notice
22/05/2009 8:57
Dernière modification de la notice
20/08/2019 16:08
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