Short-term culture of spleen lymphocytes for chromosome analysis of small rodents.

Details

Serval ID
serval:BIB_E46C2F7C1B44
Type
Article: article from journal or magazin.
Collection
Publications
Title
Short-term culture of spleen lymphocytes for chromosome analysis of small rodents.
Journal
Genetica
Author(s)
Jotterand Bellomo M., Mühlematter D., Nabholz M.
ISSN
0016-6707
Publication state
Published
Issued date
1990
Volume
83
Number
1
Pages
51-60
Language
english
Abstract
In order to improve the methods for the cytogenetic analysis of small rodents with regard to metaphase number, quality of chromosome resolution and duration of cultivation, a short-term culture technique from spleen cells, based on the capacity of Concanavalin A supernatant to stimulate the proliferation of normal T lymphocytes, was developed. Protocols of culture and chromosome preparation are described in detail. This technique not only makes it possible to obtain a high number of cells in mitosis from the spleen of very small rodents in a short time, but also insures metaphases of good quality, due to optimal control of cell cycle and processing thanks to cultivation in small aliquots. Although the method applies mainly to rodents because of species specificity of interleukin 2, it can probably be easily adapted to species from other mammalian orders.
Keywords
Animals, Arvicolinae, Chromosome Banding, Chromosomes, Cytogenetics, Female, Karyotyping, Lymphocytes, Mice, Muridae, Rodentia, Spleen
Pubmed
Create date
22/05/2009 8:57
Last modification date
20/08/2019 16:08
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