Localization of mRNAs encoding Ca2+-inhibitable adenylyl cyclases along the renal tubule. Functional consequences for regulation of the cAMP content.
Détails
ID Serval
serval:BIB_E443450754DC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Localization of mRNAs encoding Ca2+-inhibitable adenylyl cyclases along the renal tubule. Functional consequences for regulation of the cAMP content.
Périodique
Journal of Biological Chemistry
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
1996
Volume
271
Numéro
32
Pages
19264-19271
Langue
anglais
Résumé
Expression of Ca2+-inhibitable types V and VI adenylyl cyclases was studied by reverse transcription-polymerase chain reaction in rat renal glomeruli and nephron segments isolated by microdissection. Quantitation of each mRNA was achieved using a mutant cRNA which differed from the wild type by substituting two bases to create a new restriction site in the corresponding cDNA. Type VI mRNA was present all along the nephron but was more abundant in distal than in proximal segments. The expression of type V mRNA was restricted to the glomerulus and to the initial portions of the collecting duct. Expression of the Ca2+-insensitive type IV mRNA studied on the same samples was evidenced only in the glomerulus. The functional relevance of the expression of Ca2+-inhibitable isoforms was studied by measuring cAMP content in the microdissected outer medullary collecting duct which expressed both type V mRNA (2367 +/- 178 molecules/mm tubular length; n = 8) and type VI mRNA (5658 +/- 543 molecules/mm, n = 8). Agents known to increase intracellular Ca2+ in this segment induced a Ca2+-dependent inhibition on either arginine vasopressin- or glucagon-stimulated cAMP level. The characteristics of these inhibitions suggest a functional and differential expression of types V and VI adenylyl cyclases in two different cell types of the rat outer medullary collecting duct.
Mots-clé
Adenylate Cyclase/antagonists & inhibitors, Adenylate Cyclase/genetics, Animals, Arginine Vasopressin/pharmacology, Base Sequence, Calcium/pharmacology, Cyclic AMP/metabolism, DNA Primers, Glucagon/pharmacology, Kidney Medulla/drug effects, Kidney Medulla/metabolism, Kidney Tubules, Collecting/drug effects, Kidney Tubules, Collecting/metabolism, Male, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Messenger/genetics, RNA, Messenger/metabolism, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Création de la notice
24/01/2008 13:32
Dernière modification de la notice
20/08/2019 17:07