Localization of mRNAs encoding Ca2+-inhibitable adenylyl cyclases along the renal tubule. Functional consequences for regulation of the cAMP content.

Details

Serval ID
serval:BIB_E443450754DC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Localization of mRNAs encoding Ca2+-inhibitable adenylyl cyclases along the renal tubule. Functional consequences for regulation of the cAMP content.
Journal
Journal of Biological Chemistry
Author(s)
Chabardès D., Firsov D., Aarab L., Clabecq A., Bellanger A.C., Siaume-Perez S., Elalouf J.M.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Publication state
Published
Issued date
1996
Volume
271
Number
32
Pages
19264-19271
Language
english
Abstract
Expression of Ca2+-inhibitable types V and VI adenylyl cyclases was studied by reverse transcription-polymerase chain reaction in rat renal glomeruli and nephron segments isolated by microdissection. Quantitation of each mRNA was achieved using a mutant cRNA which differed from the wild type by substituting two bases to create a new restriction site in the corresponding cDNA. Type VI mRNA was present all along the nephron but was more abundant in distal than in proximal segments. The expression of type V mRNA was restricted to the glomerulus and to the initial portions of the collecting duct. Expression of the Ca2+-insensitive type IV mRNA studied on the same samples was evidenced only in the glomerulus. The functional relevance of the expression of Ca2+-inhibitable isoforms was studied by measuring cAMP content in the microdissected outer medullary collecting duct which expressed both type V mRNA (2367 +/- 178 molecules/mm tubular length; n = 8) and type VI mRNA (5658 +/- 543 molecules/mm, n = 8). Agents known to increase intracellular Ca2+ in this segment induced a Ca2+-dependent inhibition on either arginine vasopressin- or glucagon-stimulated cAMP level. The characteristics of these inhibitions suggest a functional and differential expression of types V and VI adenylyl cyclases in two different cell types of the rat outer medullary collecting duct.
Keywords
Adenylate Cyclase/antagonists & inhibitors, Adenylate Cyclase/genetics, Animals, Arginine Vasopressin/pharmacology, Base Sequence, Calcium/pharmacology, Cyclic AMP/metabolism, DNA Primers, Glucagon/pharmacology, Kidney Medulla/drug effects, Kidney Medulla/metabolism, Kidney Tubules, Collecting/drug effects, Kidney Tubules, Collecting/metabolism, Male, Molecular Sequence Data, Polymerase Chain Reaction, RNA, Messenger/genetics, RNA, Messenger/metabolism, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Create date
24/01/2008 12:32
Last modification date
20/08/2019 16:07
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