T cell receptor V beta repertoire in mice lacking endogenous mouse mammary tumor provirus.

Détails

ID Serval
serval:BIB_D8CF4D428E62
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
T cell receptor V beta repertoire in mice lacking endogenous mouse mammary tumor provirus.
Périodique
European Journal of Immunology
Auteur(s)
Braun M.Y., Jouvin-Marche E., Marche P.N., MacDonald H.R., Acha-Orbea H.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Statut éditorial
Publié
Date de publication
1995
Peer-reviewed
Oui
Volume
25
Numéro
3
Pages
857-862
Langue
anglais
Résumé
When endogenous mouse mammary tumor virus (MMTV) superantigens (SAg) are expressed in the first weeks of life an efficient thymic deletion of T cells expressing MMTV SAg-reactive T cell receptor (TcR) V beta segments is observed. As most inbred mouse strains and wild mice contain integrated MMTV DNA, knowing the precise extent of MMTV influence on T cell development is required in order to study T cell immunobiology in the mouse. In this report, backcross breeding between BALB.D2 (Mtv-6, -7, -8 and -9) and 38CH (Mtv-) mice was carried out to obtain animals either lacking endogenous MMTV or containing a single MMTV locus, i.e. Mtv-6, -7, -8 or -9. The TcR V beta chain (TcR V beta) usage in these mice was analyzed using monoclonal antibodies specific for TcR V beta 2, V beta 3, V beta 4, V beta 5, V beta 6, V beta 7, V beta 8, V beta 11, V beta 12 and V beta 14 segments. Both Mtv-8+ mice and Mtv-9+ mice deleted TcR V beta 5+ and V beta 11+ T cells. Moreover, we also observed the deletion of TcR V beta 12+ cells by Mtv-8 and Mtv-9 products. Mtv-6+ and Mtv-7+ animals deleted TcR V beta 3+ and V beta 5+ cells, and TcR V beta 6+, V beta 7+ and V beta 8.1+ cells, respectively. Unexpectedly, TcR V beta 8.2+ cells were also deleted in some backcross mice expressing Mtv-7. TcR V beta 8.2 reactivity to Mtv-7 was shown to be brought by the 38CH strain and to result from an amino acid substitution (Asn-->Asp) in position 19 on the TcR V beta 8.2 fragment. Reactivities of BALB.D2 TcR V beta 8.2 and 38CH TcR V beta 8.2 to the exogenous infectious viruses, MMTV(SW) and MMTV(SHN), were compared. Finally, the observation of increased frequencies of TcR V beta 2+, V beta 4+ and V beta 8+ CD4+ T cell subsets in Mtv-8+ and Mtv-9+ mice, and TcR V beta 4+ CD4+ T cells in Mtv-6+ and Mtv-7+ mice, when compared with the T cell repertoire of Mtv- mice, is consistent with the possibility that MMTV products contribute to positive selection of T cells.
Mots-clé
Alleles, Amino Acid Sequence, Animals, Antibodies, Monoclonal/immunology, Base Sequence, Blotting, Southern, Cloning, Molecular, Flow Cytometry, Mammary Tumor Virus, Mouse/immunology, Mice, Mice, Inbred BALB C, Mice, Inbred Strains, Mice, Mutant Strains, Molecular Sequence Data, Polymerase Chain Reaction, Proviruses/immunology, Receptors, Antigen, T-Cell, alpha-beta/genetics, Receptors, Antigen, T-Cell, alpha-beta/immunology, Superantigens/immunology, T-Lymphocytes/immunology
Pubmed
Web of science
Création de la notice
24/01/2008 14:48
Dernière modification de la notice
20/08/2019 15:58
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