PCR amplification of DNA from malaria parasites on fixed and stained thick and thin blood films
Détails
ID Serval
serval:BIB_D66365B27809
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
PCR amplification of DNA from malaria parasites on fixed and stained thick and thin blood films
Périodique
Transactions of the Royal Society of Tropical Medicine and Hygiène
ISSN
0035-9203 (Print)
Statut éditorial
Publié
Date de publication
06/1997
Volume
91
Numéro
3
Pages
361-3
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May-Jun
Research Support, Non-U.S. Gov't --- Old month value: May-Jun
Résumé
Under some circumstances, polymerase chain reaction (PCR) amplification of deoxyribonucleic acid (DNA) from Plasmodium may become necessary from infections for which only blood slides are available. Established methods used for DNA preparation do not work in that case. We have developed a reliable and controlled method for DNA preparation from malaria parasites on fixed and stained blood films. 162 slides from 2 different locations, some stored for at least one year, have been analysed by PCR amplification of the polymorphic loci for MSA1 and MSA2. In 92% of microscopically positive slides, a PCR product could be detected using material derived from thick blood films. When thin blood films with scanty parasitaemia were used, a PCR product could be obtained with only 71% of samples. In all unsuccessful cases, DNA preparation was the limiting factor, which was controlled for by amplification of a control human template.
Mots-clé
Animals
Blood Pressure
Blood Specimen Collection
Child
DNA, Protozoan/genetics/*isolation & purification
Humans
Malaria, Falciparum/diagnosis
Plasmodium falciparum/*genetics
Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
28/01/2008 12:48
Dernière modification de la notice
20/08/2019 16:56