PCR amplification of DNA from malaria parasites on fixed and stained thick and thin blood films

Details

Serval ID
serval:BIB_D66365B27809
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
PCR amplification of DNA from malaria parasites on fixed and stained thick and thin blood films
Journal
Transactions of the Royal Society of Tropical Medicine and Hygiène
Author(s)
Edoh  D., Steiger  S., Genton  B., Beck  H. P.
ISSN
0035-9203 (Print)
Publication state
Published
Issued date
06/1997
Volume
91
Number
3
Pages
361-3
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May-Jun
Abstract
Under some circumstances, polymerase chain reaction (PCR) amplification of deoxyribonucleic acid (DNA) from Plasmodium may become necessary from infections for which only blood slides are available. Established methods used for DNA preparation do not work in that case. We have developed a reliable and controlled method for DNA preparation from malaria parasites on fixed and stained blood films. 162 slides from 2 different locations, some stored for at least one year, have been analysed by PCR amplification of the polymorphic loci for MSA1 and MSA2. In 92% of microscopically positive slides, a PCR product could be detected using material derived from thick blood films. When thin blood films with scanty parasitaemia were used, a PCR product could be obtained with only 71% of samples. In all unsuccessful cases, DNA preparation was the limiting factor, which was controlled for by amplification of a control human template.
Keywords
Animals Blood Pressure Blood Specimen Collection Child DNA, Protozoan/genetics/*isolation & purification Humans Malaria, Falciparum/diagnosis Plasmodium falciparum/*genetics Polymerase Chain Reaction
Pubmed
Web of science
Create date
28/01/2008 11:48
Last modification date
20/08/2019 15:56
Usage data