Identification and quantification of trihexyphenidyl and its hydroxylated metabolite by gas chromatography with nitrogen-phosphorus detection.
Détails
ID Serval
serval:BIB_D5B9648764E1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification and quantification of trihexyphenidyl and its hydroxylated metabolite by gas chromatography with nitrogen-phosphorus detection.
Périodique
Journal of Analytical Toxicology
ISSN
0146-4760[print], 0146-4760[linking]
Statut éditorial
Publié
Date de publication
1989
Volume
13
Numéro
1
Pages
47-49
Langue
anglais
Résumé
A sensitive and specific assay for the simultaneous quantification of trihexyphenidyl and its hydroxylated metabolite in plasma and urine is described. The method is based on the extraction of the drugs with an organic solvent and separation on a 3% OV-17 Chromosorb Q column in a gas chromatograph equipped with a nitrogen-phosphorus detector. The procedure employs SKF 525 A as the internal standard and requires no derivatization. The detection limit was found to be 2 ng/mL for trihexyphenidyl and 1 ng/mL for its metabolite. The precision of the assay procedure for both compounds is about 4 to 7%.
Mots-clé
Chromatography, Gas/methods, Humans, Nitrogen, Phosphorus, Trihexyphenidyl/analogs & derivatives, Trihexyphenidyl/analysis
Pubmed
Création de la notice
15/10/2010 13:56
Dernière modification de la notice
20/08/2019 16:55
Données d'usage
