Identification and quantification of trihexyphenidyl and its hydroxylated metabolite by gas chromatography with nitrogen-phosphorus detection.
Details
Serval ID
serval:BIB_D5B9648764E1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification and quantification of trihexyphenidyl and its hydroxylated metabolite by gas chromatography with nitrogen-phosphorus detection.
Journal
Journal of Analytical Toxicology
ISSN
0146-4760[print], 0146-4760[linking]
Publication state
Published
Issued date
1989
Volume
13
Number
1
Pages
47-49
Language
english
Abstract
A sensitive and specific assay for the simultaneous quantification of trihexyphenidyl and its hydroxylated metabolite in plasma and urine is described. The method is based on the extraction of the drugs with an organic solvent and separation on a 3% OV-17 Chromosorb Q column in a gas chromatograph equipped with a nitrogen-phosphorus detector. The procedure employs SKF 525 A as the internal standard and requires no derivatization. The detection limit was found to be 2 ng/mL for trihexyphenidyl and 1 ng/mL for its metabolite. The precision of the assay procedure for both compounds is about 4 to 7%.
Keywords
Chromatography, Gas/methods, Humans, Nitrogen, Phosphorus, Trihexyphenidyl/analogs & derivatives, Trihexyphenidyl/analysis
Pubmed
Create date
15/10/2010 13:56
Last modification date
20/08/2019 16:55
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