The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase.

Détails

ID Serval
serval:BIB_D5064B2F92F7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase.
Périodique
Molecular Microbiology
Auteur(s)
Reimmann C., Beyeler M., Latifi A., Winteler H., Foglino M., Lazdunski A., Haas D.
ISSN
0950-382X (Print)
ISSN-L
0950-382X
Statut éditorial
Publié
Date de publication
1997
Volume
24
Numéro
2
Pages
309-319
Langue
anglais
Résumé
The global activator GacA, a highly conserved response regulator in Gram-negative bacteria, is required for the production of exoenzymes and secondary metabolites in Pseudomonas spp. The gacA gene of Pseudomonas aeruginosa PAO1 was isolated and its role in cell-density-dependent gene expression was characterized. Mutational inactivation of gacA resulted in delayed and reduced formation of the cell-density signal N-butyryl-L-homoserine lactone (BHL), of the cognate transcriptional activator RhIR (VsmR), and of the transcriptional activator LasR, which is known to positively regulate RhIR expression. Amplification of gacA on a multicopy plasmid caused precocious and enhanced production of BHL, RhIR and LasR. In parallel, the gacA gene dosage markedly influenced the BHL/RhIR-dependent formation of the cytotoxic compounds pyocyanin and cyanide and the exoenzyme lipase. However, the concentrations of another known cell-density signal of P. aeruginosa, N-oxododecanoyl-L-homoserine lactone, did not always match BHL concentrations. A model accounting for these observations places GacA function upstream of LasR and RhIR in the complex, cell-density-dependent signal-transduction pathway regulating several exoproducts and virulence factors of P. aeruginosa via BHL.
Mots-clé
4-Butyrolactone/analogs & derivatives, 4-Butyrolactone/metabolism, Amino Acid Sequence, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Chromosome Mapping, Cloning, Molecular, Cyanides/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Gene Expression Regulation, Bacterial, Lipase/biosynthesis, Molecular Sequence Data, Plasmids, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, Pyocyanine/metabolism, Sequence Alignment, Signal Transduction/genetics, Trans-Activators/genetics, Trans-Activators/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:00
Dernière modification de la notice
20/08/2019 16:54
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