The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase.

Details

Serval ID
serval:BIB_D5064B2F92F7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase.
Journal
Molecular Microbiology
Author(s)
Reimmann C., Beyeler M., Latifi A., Winteler H., Foglino M., Lazdunski A., Haas D.
ISSN
0950-382X (Print)
ISSN-L
0950-382X
Publication state
Published
Issued date
1997
Volume
24
Number
2
Pages
309-319
Language
english
Abstract
The global activator GacA, a highly conserved response regulator in Gram-negative bacteria, is required for the production of exoenzymes and secondary metabolites in Pseudomonas spp. The gacA gene of Pseudomonas aeruginosa PAO1 was isolated and its role in cell-density-dependent gene expression was characterized. Mutational inactivation of gacA resulted in delayed and reduced formation of the cell-density signal N-butyryl-L-homoserine lactone (BHL), of the cognate transcriptional activator RhIR (VsmR), and of the transcriptional activator LasR, which is known to positively regulate RhIR expression. Amplification of gacA on a multicopy plasmid caused precocious and enhanced production of BHL, RhIR and LasR. In parallel, the gacA gene dosage markedly influenced the BHL/RhIR-dependent formation of the cytotoxic compounds pyocyanin and cyanide and the exoenzyme lipase. However, the concentrations of another known cell-density signal of P. aeruginosa, N-oxododecanoyl-L-homoserine lactone, did not always match BHL concentrations. A model accounting for these observations places GacA function upstream of LasR and RhIR in the complex, cell-density-dependent signal-transduction pathway regulating several exoproducts and virulence factors of P. aeruginosa via BHL.
Keywords
4-Butyrolactone/analogs & derivatives, 4-Butyrolactone/metabolism, Amino Acid Sequence, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Chromosome Mapping, Cloning, Molecular, Cyanides/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Gene Expression Regulation, Bacterial, Lipase/biosynthesis, Molecular Sequence Data, Plasmids, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, Pyocyanine/metabolism, Sequence Alignment, Signal Transduction/genetics, Trans-Activators/genetics, Trans-Activators/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:00
Last modification date
20/08/2019 15:54
Usage data