Harvest site influences the growth properties of adipose derived stem cells.

Détails

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Version: Final published version
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ID Serval
serval:BIB_D24AEF37D02D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Harvest site influences the growth properties of adipose derived stem cells.
Périodique
Cytotechnology
Auteur⸱e⸱s
Engels P.E., Tremp M., Kingham P.J., di Summa P.G., Largo R.D., Schaefer D.J., Kalbermatten D.F.
ISSN
0920-9069 (Print)
ISSN-L
0920-9069
Statut éditorial
Publié
Date de publication
2013
Volume
65
Numéro
3
Pages
437-445
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish. PDF type: ORIGINAL RESEARCH
Résumé
The therapeutic potential of adult stem cells may become a relevant option in clinical care in the future. In hand and plastic surgery, cell therapy might be used to enhance nerve regeneration and help surgeons and clinicians to repair debilitating nerve injuries. Adipose-derived stem cells (ASCs) are found in abundant quantities and can be harvested with a low morbidity. In order to define the optimal fat harvest location and detect any potential differences in ASC proliferation properties, we compared biopsies from different anatomical sites (inguinal, flank, pericardiac, omentum, neck) in Sprague-Dawley rats. ASCs were expanded from each biopsy and a proliferation assay using different mitogenic factors, basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) was performed. Our results show that when compared with the pericardiac region, cells isolated from the inguinal, flank, omental and neck regions grow significantly better in growth medium alone. bFGF significantly enhanced the growth rate of ASCs isolated from all regions except the omentum. PDGF had minimal effect on ASC proliferation rate but increases the growth of ASCs from the neck region. Analysis of all the data suggests that ASCs from the neck region may be the ideal stem cell sources for tissue engineering approaches for the regeneration of nervous tissue.
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/04/2013 17:12
Dernière modification de la notice
14/02/2022 7:57
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