SNAREs, tethers and SM proteins: how to overcome the final barriers to membrane fusion?

Détails

Ressource 1Télécharger: BiochemicalJ_35_AM.pdf (11440.42 [Ko])
Etat: Public
Version: Author's accepted manuscript
Licence: Non spécifiée
ID Serval
serval:BIB_D23D081057C2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
SNAREs, tethers and SM proteins: how to overcome the final barriers to membrane fusion?
Périodique
The Biochemical journal
Auteur(s)
Risselada H.J., Mayer A.
ISSN
1470-8728 (Electronic)
ISSN-L
0264-6021
Statut éditorial
Publié
Date de publication
17/01/2020
Peer-reviewed
Oui
Volume
477
Numéro
1
Pages
243-258
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Résumé
Physiological membrane vesicles are built to separate reaction spaces in a stable manner, even when they accidentally collide or are kept in apposition by spatial constraints in the cell. This requires a natural resistance to fusion and mixing of their content, which originates from substantial energetic barriers to membrane fusion [1]. To facilitate intracellular membrane fusion reactions in a controlled manner, proteinaceous fusion machineries have evolved. An important open question is whether protein fusion machineries actively pull the fusion reaction over the present free energy barriers, or whether they rather catalyze fusion by lowering those barriers. At first sight, fusion proteins such as SNARE complexes and viral fusion proteins appear to act as nano-machines, which mechanically transduce force to the membranes and thereby overcome the free energy barriers [2,3]. Whether fusion proteins additionally alter the free energy landscape of the fusion reaction via catalytic roles is less obvious. This is a question that we shall discuss in this review, with particular focus on the influence of the eukaryotic SNARE-dependent fusion machinery on the final step of the reaction, the formation and expansion of the fusion pore.
Mots-clé
Intracellular Membranes/metabolism, Membrane Fusion/physiology, SNARE Proteins/chemistry, SNARE Proteins/metabolism, Vacuoles/metabolism, Yeasts/metabolism, Rab-GTPases, SM proteins, SNARE proteins, fusion pore, membrane fusion
Pubmed
Web of science
Open Access
Oui
Financement(s)
Fonds national suisse / Projets / 31003A_179306
Création de la notice
23/01/2020 15:39
Dernière modification de la notice
15/01/2021 7:12
Données d'usage