SNAREs, tethers and SM proteins: how to overcome the final barriers to membrane fusion?
Details
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Version: Author's accepted manuscript
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State: Public
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_D23D081057C2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
SNAREs, tethers and SM proteins: how to overcome the final barriers to membrane fusion?
Journal
The Biochemical journal
ISSN
1470-8728 (Electronic)
ISSN-L
0264-6021
Publication state
Published
Issued date
17/01/2020
Peer-reviewed
Oui
Volume
477
Number
1
Pages
243-258
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Publication Status: ppublish
Abstract
Physiological membrane vesicles are built to separate reaction spaces in a stable manner, even when they accidentally collide or are kept in apposition by spatial constraints in the cell. This requires a natural resistance to fusion and mixing of their content, which originates from substantial energetic barriers to membrane fusion [1]. To facilitate intracellular membrane fusion reactions in a controlled manner, proteinaceous fusion machineries have evolved. An important open question is whether protein fusion machineries actively pull the fusion reaction over the present free energy barriers, or whether they rather catalyze fusion by lowering those barriers. At first sight, fusion proteins such as SNARE complexes and viral fusion proteins appear to act as nano-machines, which mechanically transduce force to the membranes and thereby overcome the free energy barriers [2,3]. Whether fusion proteins additionally alter the free energy landscape of the fusion reaction via catalytic roles is less obvious. This is a question that we shall discuss in this review, with particular focus on the influence of the eukaryotic SNARE-dependent fusion machinery on the final step of the reaction, the formation and expansion of the fusion pore.
Keywords
Intracellular Membranes/metabolism, Membrane Fusion/physiology, SNARE Proteins/chemistry, SNARE Proteins/metabolism, Vacuoles/metabolism, Yeasts/metabolism, Rab-GTPases, SM proteins, SNARE proteins, fusion pore, membrane fusion
Pubmed
Web of science
Open Access
Yes
Funding(s)
Swiss National Science Foundation / Projects / 31003A_179306
Create date
23/01/2020 16:39
Last modification date
15/01/2021 8:12