Accuracy of Sensititre YeastOne echinocandins epidemiological cut-off values for identification of FKS mutant Candida albicans and Candida glabrata: a ten year national survey of the Fungal Infection Network of Switzerland (FUNGINOS).

Détails

ID Serval
serval:BIB_CDB7569EE916
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Accuracy of Sensititre YeastOne echinocandins epidemiological cut-off values for identification of FKS mutant Candida albicans and Candida glabrata: a ten year national survey of the Fungal Infection Network of Switzerland (FUNGINOS).
Périodique
Clinical microbiology and infection
Auteur(s)
Kritikos A., Neofytos D., Khanna N., Schreiber P.W., Boggian K., Bille J., Schrenzel J., Mühlethaler K., Zbinden R., Bruderer T., Goldenberger D., Pfyffer G., Conen A., Van Delden C., Zimmerli S., Sanglard D., Bachmann D., Marchetti O., Lamoth F.
Collaborateur(s)
Fungal Infection Network of Switzerland (FUNGINOS)
Contributeur(s)
Bregenzer T., Conen A., Flückiger U., Khanna N., Orasch C., Heininger U., Franciolli M., Damonti L., Zimmerli S., Rothen M., Zellweger C., Tarr P., Fleisch F., Chuard C., Erard V., Emonet S., Garbino J., van Delden C., Genne D., Bochud P., Calandra T., Lamoth F., Marchetti O., Chave J., Graber P., Monotti R., Regionale O., Bernasconi E., Civico O., Rossi M., Krause M., Piso R., Bally F., Troillet N., Boggian K., Eich G., Gubler J., Fehr J., Imhof A., Ruef C., Berger C., Fankhauser H., Heinzer I., Frei R., Hertel R., Dolina M., Petrini O., Dubuis O., Mühlethaler K., Graf S., Risch M., Ritzler E., Fracheboud D., Schrenzel J., Rohner P., Lienhardt R., Bille J., Andreutti-Zaugg C., Gallusser A., Pfyffer G., Herzog K., Schibli U., Tissière L., Bruderer T., Schultze D., Zbinden R.
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Statut éditorial
Publié
Date de publication
11/2018
Peer-reviewed
Oui
Volume
24
Numéro
11
Pages
1214.e1-1214.e4
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Echinocandins represent the first-line treatment of candidaemia. Acquired echinocandin resistance is mainly observed among Candida albicans and Candida glabrata and is associated with FKS hotspot mutations. The commercial Sensititre YeastOne™ (SYO) kit is widely used for antifungal susceptibility testing, but interpretive clinical breakpoints are not well defined. We determined echinocandins epidemiological cut-off values (ECV) for C. albicans/glabrata tested by SYO and assessed their ability to identify FKS mutants in a national survey of candidaemia.
Bloodstream isolates of C. albicans and C. glabrata were collected in 25 Swiss hospitals from 2004 to 2013 and tested by SYO. FKS hotspot sequencing was performed for isolates with an MIC≥ECV for any echinocandin.
In all, 1277 C. albicans and 347 C. glabrata were included. ECV 97.5% of caspofungin, anidulafungin and micafungin were 0.12, 0.06 and 0.03 μg/mL for C. albicans, and 0.25, 0.12 and 0.03 μg/mL for C. glabrata, respectively. FKS hotspot sequencing was performed for 70 isolates. No mutation was found in the 52 'limit wild-type' isolates (MIC=ECV for at least one echinocandin). Among the 18 'non-wild-type' isolates (MIC>ECV for at least one echinocandin), FKS mutations were recovered in the only two isolates with MIC>ECV for all three echinocandins, but not in those exhibiting a 'non-wild-type' phenotype for only one or two echinocandins.
This 10-year nationwide survey showed that the rate of echinocandin resistance among C. albicans and C. glabrata remains low in Switzerland despite increased echinocandin use. SYO-ECV could discriminate FKS mutants from wild-type isolates tested by SYO in this population.
Mots-clé
Antifungal Agents/pharmacology, Candida albicans/genetics, Candida glabrata, Candidiasis/microbiology, Drug Resistance, Fungal, Echinocandins/administration & dosage, Echinocandins/pharmacology, Humans, Microbial Sensitivity Tests, Mutation, Population Surveillance, Switzerland/epidemiology, Anidulafungin, Antifungal susceptibility testing, Candidaemia, Caspofungin, FKS hotspot, Micafungin, Minimal inhibitory concentration
Pubmed
Web of science
Création de la notice
20/07/2018 16:33
Dernière modification de la notice
20/08/2019 16:48
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