Accuracy of Sensititre YeastOne echinocandins epidemiological cut-off values for identification of FKS mutant Candida albicans and Candida glabrata: a ten year national survey of the Fungal Infection Network of Switzerland (FUNGINOS).
Details
Serval ID
serval:BIB_CDB7569EE916
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Accuracy of Sensititre YeastOne echinocandins epidemiological cut-off values for identification of FKS mutant Candida albicans and Candida glabrata: a ten year national survey of the Fungal Infection Network of Switzerland (FUNGINOS).
Journal
Clinical microbiology and infection
Working group(s)
Fungal Infection Network of Switzerland (FUNGINOS)
Contributor(s)
Bregenzer T., Conen A., Flückiger U., Khanna N., Orasch C., Heininger U., Franciolli M., Damonti L., Zimmerli S., Rothen M., Zellweger C., Tarr P., Fleisch F., Chuard C., Erard V., Emonet S., Garbino J., van Delden C., Genne D., Bochud P., Calandra T., Lamoth F., Marchetti O., Chave J., Graber P., Monotti R., Regionale O., Bernasconi E., Civico O., Rossi M., Krause M., Piso R., Bally F., Troillet N., Boggian K., Eich G., Gubler J., Fehr J., Imhof A., Ruef C., Berger C., Fankhauser H., Heinzer I., Frei R., Hertel R., Dolina M., Petrini O., Dubuis O., Mühlethaler K., Graf S., Risch M., Ritzler E., Fracheboud D., Schrenzel J., Rohner P., Lienhardt R., Bille J., Andreutti-Zaugg C., Gallusser A., Pfyffer G., Herzog K., Schibli U., Tissière L., Bruderer T., Schultze D., Zbinden R.
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Publication state
Published
Issued date
11/2018
Peer-reviewed
Oui
Volume
24
Number
11
Pages
1214.e1-1214.e4
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Echinocandins represent the first-line treatment of candidaemia. Acquired echinocandin resistance is mainly observed among Candida albicans and Candida glabrata and is associated with FKS hotspot mutations. The commercial Sensititre YeastOne™ (SYO) kit is widely used for antifungal susceptibility testing, but interpretive clinical breakpoints are not well defined. We determined echinocandins epidemiological cut-off values (ECV) for C. albicans/glabrata tested by SYO and assessed their ability to identify FKS mutants in a national survey of candidaemia.
Bloodstream isolates of C. albicans and C. glabrata were collected in 25 Swiss hospitals from 2004 to 2013 and tested by SYO. FKS hotspot sequencing was performed for isolates with an MIC≥ECV for any echinocandin.
In all, 1277 C. albicans and 347 C. glabrata were included. ECV 97.5% of caspofungin, anidulafungin and micafungin were 0.12, 0.06 and 0.03 μg/mL for C. albicans, and 0.25, 0.12 and 0.03 μg/mL for C. glabrata, respectively. FKS hotspot sequencing was performed for 70 isolates. No mutation was found in the 52 'limit wild-type' isolates (MIC=ECV for at least one echinocandin). Among the 18 'non-wild-type' isolates (MIC>ECV for at least one echinocandin), FKS mutations were recovered in the only two isolates with MIC>ECV for all three echinocandins, but not in those exhibiting a 'non-wild-type' phenotype for only one or two echinocandins.
This 10-year nationwide survey showed that the rate of echinocandin resistance among C. albicans and C. glabrata remains low in Switzerland despite increased echinocandin use. SYO-ECV could discriminate FKS mutants from wild-type isolates tested by SYO in this population.
Bloodstream isolates of C. albicans and C. glabrata were collected in 25 Swiss hospitals from 2004 to 2013 and tested by SYO. FKS hotspot sequencing was performed for isolates with an MIC≥ECV for any echinocandin.
In all, 1277 C. albicans and 347 C. glabrata were included. ECV 97.5% of caspofungin, anidulafungin and micafungin were 0.12, 0.06 and 0.03 μg/mL for C. albicans, and 0.25, 0.12 and 0.03 μg/mL for C. glabrata, respectively. FKS hotspot sequencing was performed for 70 isolates. No mutation was found in the 52 'limit wild-type' isolates (MIC=ECV for at least one echinocandin). Among the 18 'non-wild-type' isolates (MIC>ECV for at least one echinocandin), FKS mutations were recovered in the only two isolates with MIC>ECV for all three echinocandins, but not in those exhibiting a 'non-wild-type' phenotype for only one or two echinocandins.
This 10-year nationwide survey showed that the rate of echinocandin resistance among C. albicans and C. glabrata remains low in Switzerland despite increased echinocandin use. SYO-ECV could discriminate FKS mutants from wild-type isolates tested by SYO in this population.
Keywords
Antifungal Agents/pharmacology, Candida albicans/genetics, Candida glabrata, Candidiasis/microbiology, Drug Resistance, Fungal, Echinocandins/administration & dosage, Echinocandins/pharmacology, Humans, Microbial Sensitivity Tests, Mutation, Population Surveillance, Switzerland/epidemiology, Anidulafungin, Antifungal susceptibility testing, Candidaemia, Caspofungin, FKS hotspot, Micafungin, Minimal inhibitory concentration
Pubmed
Web of science
Create date
20/07/2018 16:33
Last modification date
25/02/2022 7:36
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