Differentially regulated promoters for antigen expression in Salmonella vaccine strains.

Détails

ID Serval
serval:BIB_CBE4BD411F79
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differentially regulated promoters for antigen expression in Salmonella vaccine strains.
Périodique
Vaccine
Auteur(s)
Stojanov M., Besançon H., Snäkä T., Nardelli-Haefliger D., Curtiss R., Baud D.
ISSN
1873-2518 (Electronic)
ISSN-L
0264-410X
Statut éditorial
Publié
Date de publication
27/05/2020
Peer-reviewed
Oui
Volume
38
Numéro
26
Pages
4154-4161
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
In most attenuated Salmonella enterica vaccines, heterologous antigens are expressed under the control of strong inducible promoters to ensure a high level of synthesis. Although high expression levels of the antigen can improve the immunogenicity of the vaccine, they might be toxic to the Salmonella carrier. Expression problems could be avoided by the use of promoters with specific characteristics with respect to strength and timing of expression. To study the expression of ten selected promoters, translational promoter-green fluorescent protein (GFP) fusions were analyzed in three attenuated Salmonella strains, Ty21a, SL3261 and PhoP <sup>C</sup> . Promoter expression was evaluated both in vitro and in intracellular conditions using flow cytometry and confocal microscopy, with specific focus on the levels and timing of expression. We identified one major candidate promoter (Pasr) that could be used to express antigens specifically during in vivo conditions, without impairing bacterial growth during in vitro vaccine production.
Mots-clé
Antigen expression, Promoter, Salmonella, Vaccine
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/06/2020 15:18
Dernière modification de la notice
15/09/2020 6:26
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