Differentially regulated promoters for antigen expression in Salmonella vaccine strains.

Details

Serval ID
serval:BIB_CBE4BD411F79
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differentially regulated promoters for antigen expression in Salmonella vaccine strains.
Journal
Vaccine
Author(s)
Stojanov M., Besançon H., Snäkä T., Nardelli-Haefliger D., Curtiss R., Baud D.
ISSN
1873-2518 (Electronic)
ISSN-L
0264-410X
Publication state
Published
Issued date
27/05/2020
Peer-reviewed
Oui
Volume
38
Number
26
Pages
4154-4161
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
In most attenuated Salmonella enterica vaccines, heterologous antigens are expressed under the control of strong inducible promoters to ensure a high level of synthesis. Although high expression levels of the antigen can improve the immunogenicity of the vaccine, they might be toxic to the Salmonella carrier. Expression problems could be avoided by the use of promoters with specific characteristics with respect to strength and timing of expression. To study the expression of ten selected promoters, translational promoter-green fluorescent protein (GFP) fusions were analyzed in three attenuated Salmonella strains, Ty21a, SL3261 and PhoP <sup>C</sup> . Promoter expression was evaluated both in vitro and in intracellular conditions using flow cytometry and confocal microscopy, with specific focus on the levels and timing of expression. We identified one major candidate promoter (Pasr) that could be used to express antigens specifically during in vivo conditions, without impairing bacterial growth during in vitro vaccine production.
Keywords
Antigen expression, Promoter, Salmonella, Vaccine
Pubmed
Web of science
Open Access
Yes
Create date
16/06/2020 15:18
Last modification date
15/09/2020 6:26
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