Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection.

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_CA7B924CBC15
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection.
Périodique
Diagnostic microbiology and infectious disease
Auteur⸱e⸱s
Masseron A., Poirel L., Falgenhauer L., Imirzalioglu C., Kessler J., Chakraborty T., Nordmann P.
ISSN
1879-0070 (Electronic)
ISSN-L
0732-8893
Statut éditorial
Publié
Date de publication
07/2020
Peer-reviewed
Oui
Volume
97
Numéro
3
Pages
115059
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
OXA-244 is a derivative of OXA-48 showing weaker carbapenemase activity, compromising the detection of corresponding producers in clinical laboratories. Since 2017, the Swiss National Reference Center for Emerging Antibiotic Resistance noticed an increased identification of OXA-244-producing Escherichia coli (n=15) within the country. Different methods (biochemical and immunoassay tests, screening culture media) were tested for the detection of OXA-244 producers. Whole genome sequencing was used to investigate the genetic relatedness between the isolates and the genetic structures at the origin of the acquisition of the bla <sub>OXA-244</sub> gene. The mSuperCARBA® medium and the NG-Test CARBA5 assay were found to be suitable tools for detecting all OXA-244-producing isolates. Other selective media did not perform optimally. Among the fifteen strains, five sequence types were identified, with ST38 being predominant. The bla <sub>OXA-244</sub> gene was located on the chromosome for all isolates. Overall, detection of OXA-244 producers is challenging and specific guidelines must be followed.
Mots-clé
Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Proteins/immunology, Bacterial Proteins/metabolism, Escherichia coli/drug effects, Escherichia coli/enzymology, Escherichia coli/genetics, Escherichia coli/isolation & purification, Escherichia coli Infections/diagnosis, Escherichia coli Infections/epidemiology, Escherichia coli Infections/microbiology, Genome, Bacterial/genetics, Humans, Immunoassay, Microbial Sensitivity Tests, Switzerland/epidemiology, beta-Lactamases/genetics, beta-Lactamases/immunology, beta-Lactamases/metabolism, beta-Lactams/pharmacology, Carbapenemase, Detection, OXA-244, OXA-48
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/06/2020 14:43
Dernière modification de la notice
06/04/2024 6:35
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