Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection.
Details
Serval ID
serval:BIB_CA7B924CBC15
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection.
Journal
Diagnostic microbiology and infectious disease
ISSN
1879-0070 (Electronic)
ISSN-L
0732-8893
Publication state
Published
Issued date
07/2020
Peer-reviewed
Oui
Volume
97
Number
3
Pages
115059
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
OXA-244 is a derivative of OXA-48 showing weaker carbapenemase activity, compromising the detection of corresponding producers in clinical laboratories. Since 2017, the Swiss National Reference Center for Emerging Antibiotic Resistance noticed an increased identification of OXA-244-producing Escherichia coli (n=15) within the country. Different methods (biochemical and immunoassay tests, screening culture media) were tested for the detection of OXA-244 producers. Whole genome sequencing was used to investigate the genetic relatedness between the isolates and the genetic structures at the origin of the acquisition of the bla <sub>OXA-244</sub> gene. The mSuperCARBA® medium and the NG-Test CARBA5 assay were found to be suitable tools for detecting all OXA-244-producing isolates. Other selective media did not perform optimally. Among the fifteen strains, five sequence types were identified, with ST38 being predominant. The bla <sub>OXA-244</sub> gene was located on the chromosome for all isolates. Overall, detection of OXA-244 producers is challenging and specific guidelines must be followed.
Keywords
Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Proteins/immunology, Bacterial Proteins/metabolism, Escherichia coli/drug effects, Escherichia coli/enzymology, Escherichia coli/genetics, Escherichia coli/isolation & purification, Escherichia coli Infections/diagnosis, Escherichia coli Infections/epidemiology, Escherichia coli Infections/microbiology, Genome, Bacterial/genetics, Humans, Immunoassay, Microbial Sensitivity Tests, Switzerland/epidemiology, beta-Lactamases/genetics, beta-Lactamases/immunology, beta-Lactamases/metabolism, beta-Lactams/pharmacology, Carbapenemase, Detection, OXA-244, OXA-48
Pubmed
Web of science
Open Access
Yes
Create date
15/06/2020 15:43
Last modification date
06/04/2024 7:35