Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging.
Détails
Document(s) secondaire(s)
Télécharger: 1-s2.0-S2589004220311834-main.pdf (2514.63 [Ko])
Etat: Public
Version: Final published version
Licence: Non spécifiée
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_C5B445E71AF6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging.
Périodique
iScience
ISSN
2589-0042 (Electronic)
ISSN-L
2589-0042
Statut éditorial
Publié
Date de publication
22/01/2021
Peer-reviewed
Oui
Volume
24
Numéro
1
Pages
101986
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
For in vivo multicolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development of a new click beetle luciferase mutant, CBG2, with a red-shifted color emission. When paired with NH <sub>2</sub> -NpLH2 luciferin, CBG2 (λ = 660 nm) and CBR2 (λ = 730 nm) luciferases can be used for simultaneous dual-color bioluminescence imaging in deep tissue. Using a spectral unmixing algorithm tool it is possible to distinguish each spectral contribution. Ultimately, this enzyme pair can expand the near-infrared bioluminescent toolbox to enable rapid visualization of multiple biological processes in deep tissue using a single substrate.
Mots-clé
Biological Services, Biophysics, Optical Imaging
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/02/2021 11:37
Dernière modification de la notice
08/05/2021 6:32