Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging.
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Version: Final published version
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State: Public
Version: Final published version
License: Not specified
Serval ID
serval:BIB_C5B445E71AF6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging.
Journal
iScience
ISSN
2589-0042 (Electronic)
ISSN-L
2589-0042
Publication state
Published
Issued date
22/01/2021
Peer-reviewed
Oui
Volume
24
Number
1
Pages
101986
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
For in vivo multicolor bioluminescence applications, red and near-infrared signals are desirable over shorter wavelength signals because they are not as susceptible to light attenuation by blood and tissue. Herein, we describe the development of a new click beetle luciferase mutant, CBG2, with a red-shifted color emission. When paired with NH <sub>2</sub> -NpLH2 luciferin, CBG2 (λ = 660 nm) and CBR2 (λ = 730 nm) luciferases can be used for simultaneous dual-color bioluminescence imaging in deep tissue. Using a spectral unmixing algorithm tool it is possible to distinguish each spectral contribution. Ultimately, this enzyme pair can expand the near-infrared bioluminescent toolbox to enable rapid visualization of multiple biological processes in deep tissue using a single substrate.
Keywords
Biological Services, Biophysics, Optical Imaging
Pubmed
Web of science
Open Access
Yes
Create date
19/02/2021 11:37
Last modification date
08/05/2021 6:32