Purification and characterization of murine lipopolysaccharide-binding protein

Détails

ID Serval
serval:BIB_C5382F73D7C6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Purification and characterization of murine lipopolysaccharide-binding protein
Périodique
Infection and Immunity
Auteur(s)
Gallay  P., Carrel  S., Glauser  M. P., Barras  C., Ulevitch  R. J., Tobias  P. S., Baumgartner  J. D., Heumann  D.
ISSN
0019-9567
Statut éditorial
Publié
Date de publication
02/1993
Peer-reviewed
Oui
Volume
61
Numéro
2
Pages
378-83
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb
Résumé
The serum protein lipopolysaccharide (LPS)-binding protein (LBP) seems to play an important role in regulating host responses to LPS. Complexes of LPS and LBP form in serum and stimulate monocytes, macrophages, or polymorphonuclear leukocytes after binding to CD14. Previous reports have described the structure and properties of LBP from human and rabbit sera. Since mice are used in some experimental models of endotoxemia or gram-negative bacterial infections, information is needed about the properties of murine LBP. Murine LBP was purified by ion-exchange chromatography and high-pressure liquid chromatography; its NH2-terminal sequence (TNPGLVTRIT) was very similar to those of human and rabbit LBPs (80 to 90% amino acid identity). Murine LBP resembled LBPs from other species in that it promoted the binding of LPS to monocytes and enhanced the sensitivity of monocytes to LPS at least 100-fold. Mouse LBP, like rabbit and human LBPs, was found to be an acute-phase protein. Further in vivo studies with mice and anti-CD14 or anti-LBP reagents should help determine the role of LBP in response to LPS challenges.
Mots-clé
Acute-Phase Proteins/analysis/*isolation & purification/physiology Amino Acid Sequence Animals Antigens, CD/metabolism Antigens, CD14 Antigens, Differentiation, Myelomonocytic/metabolism Carrier Proteins/analysis/*isolation & purification/physiology Cells, Cultured Humans Hydrogen-Ion Concentration Lipopolysaccharides/metabolism *Membrane Glycoproteins Mice Mice, Inbred Strains Molecular Sequence Data Monocytes/metabolism Rabbits Temperature
Pubmed
Web of science
Création de la notice
19/01/2008 19:12
Dernière modification de la notice
20/08/2019 16:40
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