Purification and characterization of murine lipopolysaccharide-binding protein
Details
Serval ID
serval:BIB_C5382F73D7C6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Purification and characterization of murine lipopolysaccharide-binding protein
Journal
Infection and Immunity
ISSN
0019-9567
Publication state
Published
Issued date
02/1993
Peer-reviewed
Oui
Volume
61
Number
2
Pages
378-83
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb
Abstract
The serum protein lipopolysaccharide (LPS)-binding protein (LBP) seems to play an important role in regulating host responses to LPS. Complexes of LPS and LBP form in serum and stimulate monocytes, macrophages, or polymorphonuclear leukocytes after binding to CD14. Previous reports have described the structure and properties of LBP from human and rabbit sera. Since mice are used in some experimental models of endotoxemia or gram-negative bacterial infections, information is needed about the properties of murine LBP. Murine LBP was purified by ion-exchange chromatography and high-pressure liquid chromatography; its NH2-terminal sequence (TNPGLVTRIT) was very similar to those of human and rabbit LBPs (80 to 90% amino acid identity). Murine LBP resembled LBPs from other species in that it promoted the binding of LPS to monocytes and enhanced the sensitivity of monocytes to LPS at least 100-fold. Mouse LBP, like rabbit and human LBPs, was found to be an acute-phase protein. Further in vivo studies with mice and anti-CD14 or anti-LBP reagents should help determine the role of LBP in response to LPS challenges.
Keywords
Acute-Phase Proteins/analysis/*isolation & purification/physiology
Amino Acid Sequence
Animals
Antigens, CD/metabolism
Antigens, CD14
Antigens, Differentiation, Myelomonocytic/metabolism
Carrier Proteins/analysis/*isolation & purification/physiology
Cells, Cultured
Humans
Hydrogen-Ion Concentration
Lipopolysaccharides/metabolism
*Membrane Glycoproteins
Mice
Mice, Inbred Strains
Molecular Sequence Data
Monocytes/metabolism
Rabbits
Temperature
Pubmed
Web of science
Create date
19/01/2008 18:12
Last modification date
20/08/2019 15:40