Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium

Détails

Ressource 1Télécharger: fichier (2044.42 [Ko])
Etat: Public
Version: Final published version
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
ID Serval
serval:BIB_C43912DEA8D5
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium
Périodique
Fems Microbiology Ecology
Auteur⸱e⸱s
Simon A., Bindschedler S., Job D., Wick L.Y., Filippidou S., Kooli W.M., Verrecchia E.P., Junier P.
ISSN
1574-6941 (Online)
ISSN-L
0168-6496
Statut éditorial
Publié
Date de publication
2015
Volume
91
Numéro
11
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Fungi and bacteria form various associations that are central to numerous environmental processes. In the so-called fungal highway, bacteria disperse along fungal mycelium. We developed a novel tool for the in situ isolation of bacteria moving along fungal hyphae as well as for the recovery of fungi potentially involved in dispersal, both of which are attracted towards a target culture medium. We present the validation and the results of the first in situ test. Couples of fungi and bacteria were isolated from soil. Amongst the enriched organisms, we identified several species of fast-growing fungi (Fusarium sp. and Chaetomium sp.), as well as various potentially associated bacterial groups, including Variovorax soli, Olivibacter soli, Acinetobacter calcoaceticus, and several species of the genera Stenotrophomonas, Achromobacter and Ochrobactrum. Migration of bacteria along fungal hyphae across a discontinuous medium was confirmed in most of the cases. Although the majority of the bacteria for which migration was confirmed were also positive for flagellar motility, not all motile bacteria dispersed using their potential fungal partner. In addition, the importance of hydrophobicity of the fungal mycelial surface was confirmed. Future applications of the columns include targeting different types of microorganisms and their interactions, either by enrichment or by state of the art molecular biological methods.
Mots-clé
Bacteria/classification, Bacteria/isolation & purification, Fungi/classification, Fungi/cytology, Fungi/growth & development, Hyphae/metabolism, Hyphae/ultrastructure, Pseudomonas putida/isolation & purification, Pseudomonas putida/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/07/2018 9:00
Dernière modification de la notice
21/11/2022 8:19
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