Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium

Details

Ressource 1Download: serval:BIB_C43912DEA8D5.P001 (2044.42 [Ko])
State: Public
Version: author
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_C43912DEA8D5
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Exploiting the fungal highway: development of a novel tool for the in situ isolation of bacteria migrating along fungal mycelium
Journal
Fems Microbiology Ecology
Author(s)
Simon A., Bindschedler S., Job D., Wick L.Y., Filippidou S., Kooli W.M., Verrecchia E.P., Junier P.
ISSN
1574-6941 (Electronic)
ISSN-L
0168-6496
Publication state
Published
Issued date
2015
Volume
91
Number
11
Pages
X
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Fungi and bacteria form various associations that are central to numerous environmental processes. In the so-called fungal highway, bacteria disperse along fungal mycelium. We developed a novel tool for the in situ isolation of bacteria moving along fungal hyphae as well as for the recovery of fungi potentially involved in dispersal, both of which are attracted towards a target culture medium. We present the validation and the results of the first in situ test. Couples of fungi and bacteria were isolated from soil. Amongst the enriched organisms, we identified several species of fast-growing fungi (Fusarium sp. and Chaetomium sp.), as well as various potentially associated bacterial groups, including Variovorax soli, Olivibacter soli, Acinetobacter calcoaceticus, and several species of the genera Stenotrophomonas, Achromobacter and Ochrobactrum. Migration of bacteria along fungal hyphae across a discontinuous medium was confirmed in most of the cases. Although the majority of the bacteria for which migration was confirmed were also positive for flagellar motility, not all motile bacteria dispersed using their potential fungal partner. In addition, the importance of hydrophobicity of the fungal mycelial surface was confirmed. Future applications of the columns include targeting different types of microorganisms and their interactions, either by enrichment or by state of the art molecular biological methods.
Keywords
Bacteria/classification, Bacteria/isolation & purification, Fungi/classification, Fungi/cytology, Fungi/growth & development, Hyphae/metabolism, Hyphae/ultrastructure, Pseudomonas putida/isolation & purification, Pseudomonas putida/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
13/07/2018 10:00
Last modification date
25/09/2019 7:10
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