Cell cycle-dependent and independent mating blocks ensure fungal zygote survival and ploidy maintenance.

Détails

Ressource 1Télécharger: journal.pbio.3001067.pdf (5343.67 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_C2F7AF4FFA12
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cell cycle-dependent and independent mating blocks ensure fungal zygote survival and ploidy maintenance.
Périodique
PLoS biology
Auteur⸱e⸱s
Vještica A., Bérard M., Liu G., Merlini L., Nkosi P.J., Martin S.G.
ISSN
1545-7885 (Electronic)
ISSN-L
1544-9173
Statut éditorial
Publié
Date de publication
01/2021
Peer-reviewed
Oui
Volume
19
Numéro
1
Pages
e3001067
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
To ensure genome stability, sexually reproducing organisms require that mating brings together exactly 2 haploid gametes and that meiosis occurs only in diploid zygotes. In the fission yeast Schizosaccharomyces pombe, fertilization triggers the Mei3-Pat1-Mei2 signaling cascade, which represses subsequent mating and initiates meiosis. Here, we establish a degron system to specifically degrade proteins postfusion and demonstrate that mating blocks not only safeguard zygote ploidy but also prevent lysis caused by aberrant fusion attempts. Using long-term imaging and flow-cytometry approaches, we identify previously unrecognized and independent roles for Mei3 and Mei2 in zygotes. We show that Mei3 promotes premeiotic S-phase independently of Mei2 and that cell cycle progression is both necessary and sufficient to reduce zygotic mating behaviors. Mei2 not only imposes the meiotic program and promotes the meiotic cycle, but also blocks mating behaviors independently of Mei3 and cell cycle progression. Thus, we find that fungi preserve zygote ploidy and survival by at least 2 mechanisms where the zygotic fate imposed by Mei2 and the cell cycle reentry triggered by Mei3 synergize to prevent zygotic mating.
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/01/2021 13:26
Dernière modification de la notice
07/07/2021 6:12
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