Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation.

Détails

Ressource 1Télécharger: 31978041_BIB_BF729C36E100.pdf (3572.45 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_BF729C36E100
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation.
Périodique
PLoS genetics
Auteur(s)
Dold A., Han H., Liu N., Hildebrandt A., Brüggemann M., Rücklé C., Hänel H., Busch A., Beli P., Zarnack K., König J., Roignant J.Y., Lasko P.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Statut éditorial
Publié
Date de publication
01/2020
Peer-reviewed
Oui
Volume
16
Numéro
1
Pages
e1008581
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Makorins are evolutionary conserved proteins that contain C3H-type zinc finger modules and a RING E3 ubiquitin ligase domain. In Drosophila, maternal Makorin 1 (Mkrn1) has been linked to embryonic patterning but the mechanism remained unsolved. Here, we show that Mkrn1 is essential for axis specification and pole plasm assembly by translational activation of oskar (osk). We demonstrate that Mkrn1 interacts with poly(A) binding protein (pAbp) and binds specifically to osk 3' UTR in a region adjacent to A-rich sequences. Using Drosophila S2R+ cultured cells we show that this binding site overlaps with a Bruno1 (Bru1) responsive element (BREs) that regulates osk translation. We observe increased association of the translational repressor Bru1 with osk mRNA upon depletion of Mkrn1, indicating that both proteins compete for osk binding. Consistently, reducing Bru1 dosage partially rescues viability and Osk protein level in ovaries from Mkrn1 females. We conclude that Mkrn1 controls embryonic patterning and germ cell formation by specifically activating osk translation, most likely by competing with Bru1 to bind to osk 3' UTR.
Mots-clé
3' Untranslated Regions, Animals, Body Patterning, Cell Line, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila melanogaster, Female, Gene Expression Regulation, Developmental, Humans, Ovary/metabolism, Protein Binding, RNA-Binding Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2020 17:59
Dernière modification de la notice
15/01/2021 8:11
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