Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_BF729C36E100
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation.
Journal
PLoS genetics
Author(s)
Dold A., Han H., Liu N., Hildebrandt A., Brüggemann M., Rücklé C., Hänel H., Busch A., Beli P., Zarnack K., König J., Roignant J.Y., Lasko P.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Publication state
Published
Issued date
01/2020
Peer-reviewed
Oui
Volume
16
Number
1
Pages
e1008581
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Makorins are evolutionary conserved proteins that contain C3H-type zinc finger modules and a RING E3 ubiquitin ligase domain. In Drosophila, maternal Makorin 1 (Mkrn1) has been linked to embryonic patterning but the mechanism remained unsolved. Here, we show that Mkrn1 is essential for axis specification and pole plasm assembly by translational activation of oskar (osk). We demonstrate that Mkrn1 interacts with poly(A) binding protein (pAbp) and binds specifically to osk 3' UTR in a region adjacent to A-rich sequences. Using Drosophila S2R+ cultured cells we show that this binding site overlaps with a Bruno1 (Bru1) responsive element (BREs) that regulates osk translation. We observe increased association of the translational repressor Bru1 with osk mRNA upon depletion of Mkrn1, indicating that both proteins compete for osk binding. Consistently, reducing Bru1 dosage partially rescues viability and Osk protein level in ovaries from Mkrn1 females. We conclude that Mkrn1 controls embryonic patterning and germ cell formation by specifically activating osk translation, most likely by competing with Bru1 to bind to osk 3' UTR.
Keywords
3' Untranslated Regions, Animals, Body Patterning, Cell Line, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila melanogaster, Female, Gene Expression Regulation, Developmental, Humans, Ovary/metabolism, Protein Binding, RNA-Binding Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2020 16:59
Last modification date
15/01/2021 7:11
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