Comparative Transcriptomics Highlights New Features of the Iron Starvation Response in the Human Pathogen Candida Glabrata

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Ressource 1Télécharger: Benchouaia et al. - 2018 - Comparative Transcriptomics Highlights New Feature.pdf (2147.39 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_B972636DBFD4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparative Transcriptomics Highlights New Features of the Iron Starvation Response in the Human Pathogen Candida Glabrata
Périodique
Frontiers in Microbiology
Auteur(s)
Benchouaia Médine, Ripoche Hugues, Sissoko Mariam, Thiébaut Antonin, Merhej Jawad, Delaveau Thierry, Fasseu Laure, Benaissa Sabrina, Lorieux Geneviève, Jourdren Laurent, Le Crom Stéphane, Lelandais Gaëlle, Corel Eduardo, Devaux Frédéric
ISSN
1664-302X
Statut éditorial
Publié
Date de publication
2018
Volume
9
Pages
2689
Langue
anglais
Résumé
In this work, we used comparative transcriptomics to identify regulatory outliers (ROs) in the human pathogen Candida glabrata. ROs are genes that have very different expression patterns compared to their orthologs in other species. From comparative transcriptome analyses of the response of eight yeast species to toxic doses of selenite, a pleiotropic stress inducer, we identified 38 ROs in C. glabrata. Using transcriptome analyses of C. glabrata response to five different stresses, we pointed out five ROs which were more particularly responsive to iron starvation, a process which is very important for C. glabrata virulence. Global chromatin Immunoprecipitation and gene profiling analyses showed that four of these genes are actually new targets of the iron starvation responsive Aft2 transcription factor in C. glabrata. Two of them (HBS1 and DOM34b) are required for C. glabrata optimal growth in iron limited conditions. In S. cerevisiae, the orthologs of these two genes are involved in ribosome rescue by the NO GO decay (NGD) pathway. Hence, our results suggest a specific contribution of NGD co-factors to the C. glabrata adaptation to iron starvation.
Mots-clé
yeast,ChIP-seq,evolution,Aft,NO GO decay,Aft transcription factors,NO GO Decay
Pubmed
Création de la notice
22/01/2020 13:58
Dernière modification de la notice
23/01/2020 6:26
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