Comparative Transcriptomics Highlights New Features of the Iron Starvation Response in the Human Pathogen Candida Glabrata

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_B972636DBFD4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Comparative Transcriptomics Highlights New Features of the Iron Starvation Response in the Human Pathogen Candida Glabrata
Journal
Frontiers in Microbiology
Author(s)
Benchouaia Médine, Ripoche Hugues, Sissoko Mariam, Thiébaut Antonin, Merhej Jawad, Delaveau Thierry, Fasseu Laure, Benaissa Sabrina, Lorieux Geneviève, Jourdren Laurent, Le Crom Stéphane, Lelandais Gaëlle, Corel Eduardo, Devaux Frédéric
ISSN
1664-302X
Publication state
Published
Issued date
2018
Volume
9
Pages
2689
Language
english
Abstract
In this work, we used comparative transcriptomics to identify regulatory outliers (ROs) in the human pathogen Candida glabrata. ROs are genes that have very different expression patterns compared to their orthologs in other species. From comparative transcriptome analyses of the response of eight yeast species to toxic doses of selenite, a pleiotropic stress inducer, we identified 38 ROs in C. glabrata. Using transcriptome analyses of C. glabrata response to five different stresses, we pointed out five ROs which were more particularly responsive to iron starvation, a process which is very important for C. glabrata virulence. Global chromatin Immunoprecipitation and gene profiling analyses showed that four of these genes are actually new targets of the iron starvation responsive Aft2 transcription factor in C. glabrata. Two of them (HBS1 and DOM34b) are required for C. glabrata optimal growth in iron limited conditions. In S. cerevisiae, the orthologs of these two genes are involved in ribosome rescue by the NO GO decay (NGD) pathway. Hence, our results suggest a specific contribution of NGD co-factors to the C. glabrata adaptation to iron starvation.
Keywords
yeast,ChIP-seq,evolution,Aft,NO GO decay,Aft transcription factors,NO GO Decay
Pubmed
Create date
22/01/2020 13:58
Last modification date
23/01/2020 6:26
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