Immunological approach to hepatocellular carcinoma
Détails
ID Serval
serval:BIB_B7ABFA50FFCA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Immunological approach to hepatocellular carcinoma
Périodique
Journal of Viral Hepatitis
ISSN
1352-0504 (Print)
Statut éditorial
Publié
Date de publication
1997
Volume
4 Suppl 2
Pages
60-74
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S.
Research Support, U.S. Gov't, P.H.S.
Résumé
A library of monoclonal antibodies (MoAbs) has been produced against a human hepatocellular carcinoma (HCC) cell line designated FOCUS in order to study the antigenic properties of transformed hepatocytes. Several monoclonal antibodies (MoAbs) were initially selected for study since they bound to antigens which were overexpressed in HCC tissues compared with the adjacent uninvolved normal liver counterpart; in addition, these MoAbs revealed low level antigen expression on other normal human tissues. Subsequently, HCC cell lines were metabolically labelled and the antigens further characterized by immunoprecipitation and Western blot analysis. If the MoAb recognized a primary linear epitope on a protein, cloning was performed using a lambda GT11 cDNA expression library prepared from the FOCUS HCC cell line. These studies characterized the HCC associated antigen(s) at the molecular level. This review illustrates the value of such an experimental approach to search for and identify HCC associated antigens and emphasizes the biological properties of novel proteins may be defined and characterized by these techniques. More important, our investigations have described unique proteins that may not only be important in the pathogenesis of HCC but also demonstrates how such antigen-antibody systems may be used to develop strategies for immunotargetting and gene therapy of HCC.
Mots-clé
Animals
Antibodies, Monoclonal/immunology
Antibodies, Neoplasm/*immunology
Antigens, Neoplasm/*immunology
Carcinoma, Hepatocellular/*immunology
Cations
Cloning, Molecular
Gene Transfer Techniques
Humans
Liposomes
Mice
Mice, Nude
Mixed Function Oxygenases/genetics
Phenotype
Phosphoproteins/biosynthesis/genetics
Time Factors
Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
25/01/2008 17:06
Dernière modification de la notice
20/08/2019 16:25