Immunological approach to hepatocellular carcinoma

Details

Serval ID
serval:BIB_B7ABFA50FFCA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Immunological approach to hepatocellular carcinoma
Journal
Journal of Viral Hepatitis
Author(s)
Wands  J. R., Lavaissiere  L., Moradpour  D., de la Monte  S., Mohr  L., Nicolau  C., Tanaka  S.
ISSN
1352-0504 (Print)
Publication state
Published
Issued date
1997
Volume
4 Suppl 2
Pages
60-74
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S.
Abstract
A library of monoclonal antibodies (MoAbs) has been produced against a human hepatocellular carcinoma (HCC) cell line designated FOCUS in order to study the antigenic properties of transformed hepatocytes. Several monoclonal antibodies (MoAbs) were initially selected for study since they bound to antigens which were overexpressed in HCC tissues compared with the adjacent uninvolved normal liver counterpart; in addition, these MoAbs revealed low level antigen expression on other normal human tissues. Subsequently, HCC cell lines were metabolically labelled and the antigens further characterized by immunoprecipitation and Western blot analysis. If the MoAb recognized a primary linear epitope on a protein, cloning was performed using a lambda GT11 cDNA expression library prepared from the FOCUS HCC cell line. These studies characterized the HCC associated antigen(s) at the molecular level. This review illustrates the value of such an experimental approach to search for and identify HCC associated antigens and emphasizes the biological properties of novel proteins may be defined and characterized by these techniques. More important, our investigations have described unique proteins that may not only be important in the pathogenesis of HCC but also demonstrates how such antigen-antibody systems may be used to develop strategies for immunotargetting and gene therapy of HCC.
Keywords
Animals Antibodies, Monoclonal/immunology Antibodies, Neoplasm/*immunology Antigens, Neoplasm/*immunology Carcinoma, Hepatocellular/*immunology Cations Cloning, Molecular Gene Transfer Techniques Humans Liposomes Mice Mice, Nude Mixed Function Oxygenases/genetics Phenotype Phosphoproteins/biosynthesis/genetics Time Factors Tumor Cells, Cultured
Pubmed
Web of science
Create date
25/01/2008 16:06
Last modification date
20/08/2019 15:25
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