Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function.

Détails

ID Serval
serval:BIB_B2BA9D31B889
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Institution
Titre
Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function.
Périodique
Methods in molecular biology
Auteur(s)
Sabine A., Davis M.J., Bovay E., Petrova T.V.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
1846
Pages
97-129
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Intraluminal valves of collecting lymphatic vessels ensure unidirectional lymph transport against hydrostatic pressure gradient. Mouse mesentery harbors up to 800 valves and represents a convenient model for lymphatic valve quantification, high resolution imaging of different stages of valve development as well as for analysis of valve function. The protocol describes embryonic and postnatal mesenteric lymphatic vessel preparation for whole-mount immunofluorescent staining and visualization of valve organization, quantification of main morphological parameters such as valve size and leaflet length, and the quantitative assessment of functional properties of adult valves using back-leak and closure tests.
Mots-clé
Angiography/methods, Animals, Fluorescent Antibody Technique, Image Processing, Computer-Assisted, Lymphatic Vessels/anatomy & histology, Lymphatic Vessels/physiology, Mesentery/anatomy & histology, Mesentery/blood supply, Mice, Microscopy, Fluorescence/methods, Collecting vessel, Lymphatic valve, Mesentery
Pubmed
Création de la notice
29/10/2018 9:55
Dernière modification de la notice
20/08/2019 15:21
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