Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function
Details
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Version: Author's accepted manuscript
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UNIL restricted access
State: Public
Version: Author's accepted manuscript
License: Not specified
Serval ID
serval:BIB_B2BA9D31B889
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function
Title of the book
Methods in Molecular Biology
Publisher
Springer New York
ISBN
9781493987115
9781493987122
9781493987122
ISSN
1064-3745
1940-6029
1940-6029
ISSN-L
1064-3745
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
1846
Chapter
7
Pages
97-129
Language
english
Abstract
Intraluminal valves of collecting lymphatic vessels ensure unidirectional lymph transport against hydrostatic pressure gradient. Mouse mesentery harbors up to 800 valves and represents a convenient model for lymphatic valve quantification, high resolution imaging of different stages of valve development as well as for analysis of valve function. The protocol describes embryonic and postnatal mesenteric lymphatic vessel preparation for whole-mount immunofluorescent staining and visualization of valve organization, quantification of main morphological parameters such as valve size and leaflet length, and the quantitative assessment of functional properties of adult valves using back-leak and closure tests.
Pubmed
Web of science
Create date
29/10/2018 10:55
Last modification date
30/07/2024 7:02