Mammalian embryo comparison identifies novel pluripotency genes associated with the naïve or primed state.

Détails

Ressource 1Télécharger: 30026265_BIB_B219448F8799.pdf (8315.31 [Ko])
Etat: Public
Version: de l'auteur
Licence: CC BY 4.0
ID Serval
serval:BIB_B219448F8799
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Mammalian embryo comparison identifies novel pluripotency genes associated with the naïve or primed state.
Périodique
Biology open
Auteur(s)
Bernardo A.S., Jouneau A., Marks H., Kensche P., Kobolak J., Freude K., Hall V., Feher A., Polgar Z., Sartori C., Bock I., Louet C., Faial T., Kerstens HHD, Bouissou C., Parsonage G., Mashayekhi K., Smith J.C., Lazzari G., Hyttel P., Stunnenberg H.G., Huynen M., Pedersen R.A., Dinnyes A.
ISSN
2046-6390 (Print)
ISSN-L
2046-6390
Statut éditorial
Publié
Date de publication
17/08/2018
Peer-reviewed
Oui
Volume
7
Numéro
8
Pages
1
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, 'naïve' or 'primed', depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene expression patterns of mouse, cattle and porcine embryos at stages that harbour different types of pluripotent cells. We document conserved and divergent traits in gene expression, and identify predictor genes shared across the species that are associated with pluripotent states <i>in vivo</i> and <i>in vitro</i> Amongst these are the pluripotency-linked genes <i>Klf4</i> and <i>Lin28b</i> The novel genes discovered include naïve- ( <i>Spic, Scpep1</i> and <i>Gjb5</i> ) and primed-associated ( <i>Sema6a</i> and <i>Jakmip2</i> ) genes as well as naïve to primed transition genes ( <i>Dusp6</i> and <i>Trip6</i> ). Both <i>Gjb5</i> and <i>Dusp6</i> play a role in pluripotency since their knockdown results in differentiation and downregulation of key pluripotency genes. Our interspecies comparison revealed new insights of pluripotency, pluripotent stem cell identity and a new molecular criterion for distinguishing between pluripotent states in various species, including human.
Mots-clé
Dusp6, Gjb5, Naïve, Pluripotency, Primed, Trip6
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/08/2018 17:38
Dernière modification de la notice
30/04/2021 6:14
Données d'usage