Mammalian embryo comparison identifies novel pluripotency genes associated with the naïve or primed state.

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Serval ID
serval:BIB_B219448F8799
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mammalian embryo comparison identifies novel pluripotency genes associated with the naïve or primed state.
Journal
Biology open
Author(s)
Bernardo A.S., Jouneau A., Marks H., Kensche P., Kobolak J., Freude K., Hall V., Feher A., Polgar Z., Sartori C., Bock I., Louet C., Faial T., Kerstens HHD, Bouissou C., Parsonage G., Mashayekhi K., Smith J.C., Lazzari G., Hyttel P., Stunnenberg H.G., Huynen M., Pedersen R.A., Dinnyes A.
ISSN
2046-6390 (Print)
ISSN-L
2046-6390
Publication state
Published
Issued date
17/08/2018
Peer-reviewed
Oui
Volume
7
Number
8
Pages
1
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
During early mammalian development, transient pools of pluripotent cells emerge that can be immortalised upon stem cell derivation. The pluripotent state, 'naïve' or 'primed', depends on the embryonic stage and derivation conditions used. Here we analyse the temporal gene expression patterns of mouse, cattle and porcine embryos at stages that harbour different types of pluripotent cells. We document conserved and divergent traits in gene expression, and identify predictor genes shared across the species that are associated with pluripotent states <i>in vivo</i> and <i>in vitro</i> Amongst these are the pluripotency-linked genes <i>Klf4</i> and <i>Lin28b</i> The novel genes discovered include naïve- ( <i>Spic, Scpep1</i> and <i>Gjb5</i> ) and primed-associated ( <i>Sema6a</i> and <i>Jakmip2</i> ) genes as well as naïve to primed transition genes ( <i>Dusp6</i> and <i>Trip6</i> ). Both <i>Gjb5</i> and <i>Dusp6</i> play a role in pluripotency since their knockdown results in differentiation and downregulation of key pluripotency genes. Our interspecies comparison revealed new insights of pluripotency, pluripotent stem cell identity and a new molecular criterion for distinguishing between pluripotent states in various species, including human.
Keywords
Dusp6, Gjb5, Naïve, Pluripotency, Primed, Trip6
Pubmed
Web of science
Open Access
Yes
Create date
21/08/2018 17:38
Last modification date
30/04/2021 6:14
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