Correlation between cytotoxicity induced by Pseudomonas aeruginosa clinical isolates from acute infections and IL-1β secretion in a model of human THP-1 monocytes.

Détails

ID Serval
serval:BIB_B166B70505DE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Correlation between cytotoxicity induced by Pseudomonas aeruginosa clinical isolates from acute infections and IL-1β secretion in a model of human THP-1 monocytes.
Périodique
Pathogens and disease
Auteur⸱e⸱s
Anantharajah A., Buyck J.M., Faure E., Glupczynski Y., Rodriguez-Villalobos H., De Vos D., Pirnay J.P., Bilocq F., Guery B., Tulkens P.M., Mingeot-Leclercq M.P., Van Bambeke F.
ISSN
2049-632X (Electronic)
ISSN-L
2049-632X
Statut éditorial
Publié
Date de publication
10/2015
Peer-reviewed
Oui
Volume
73
Numéro
7
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Type III secretion system (T3SS) in Pseudomonas aeruginosa is associated with poor clinical outcome in acute infections. T3SS allows for injection of bacterial exotoxins (e.g. ExoU or ExoS) into the host cell, causing cytotoxicity. It also activates the cytosolic NLRC4 inflammasome, activating caspase-1, inducing cytotoxicity and release of mature IL-1β, which impairs bacterial clearance. In addition, flagellum-mediated motility has been suggested to also modulate inflammasome response and IL-1β release. Yet the capacity of clinical isolates to induce IL-1β release and its relation with cytotoxicity have never been investigated. Using 20 clinical isolates from acute infections with variable T3SS expression levels and human monocytes, our aim was to correlate IL-1β release with toxin expression, flagellar motility and cytotoxicity. ExoU-producing isolates caused massive cell death but minimal release of IL-1β, while those expressing T3SS but not ExoU (i.e. expressing ExoS or no toxins) induced caspase-1 activation and IL-1β release, the level of which was correlated with cytotoxicity. Both effects were prevented by a specific caspase-1 inhibitor. Flagellar motility was not correlated with cytotoxicity or IL-1β release. No apoptosis was detected. Thus, T3SS cytotoxicity is accompanied by a modification in cytokine balance for P. aeruginosa clinical isolates that do not express ExoU.
Mots-clé
Bacterial Toxins/metabolism, Cell Death/drug effects, Cell Line, Cell Survival/drug effects, Flagella/physiology, Humans, Interleukin-1beta/metabolism, Locomotion, Monocytes/drug effects, Monocytes/microbiology, Monocytes/physiology, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/isolation & purification, Pseudomonas aeruginosa/metabolism, Pseudomonas aeruginosa/physiology, ExoS, ExoU, TNF-alpha, flagellin, inflammasome, type three secretion system
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/04/2021 9:59
Dernière modification de la notice
17/07/2023 12:25
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