Correlation between cytotoxicity induced by Pseudomonas aeruginosa clinical isolates from acute infections and IL-1beta secretion in a model of human THP-1 monocytes

Details

Serval ID
serval:BIB_B166B70505DE
Type
Article: article from journal or magazin.
Collection
Publications
Title
Correlation between cytotoxicity induced by Pseudomonas aeruginosa clinical isolates from acute infections and IL-1beta secretion in a model of human THP-1 monocytes
Journal
Pathog Dis
Author(s)
Anantharajah A., Buyck J. M., Faure E., Glupczynski Y., Rodriguez-Villalobos H., De Vos D., Pirnay J. P., Bilocq F., Guery B., Tulkens P. M., Mingeot-Leclercq M. P., Van Bambeke F.
ISSN
2049-632X (Electronic)
ISSN-L
2049-632X
Publication state
Published
Issued date
10/2015
Volume
73
Number
7
Language
english
Notes
Anantharajah, Ahalieyah
Buyck, Julien M
Faure, Emmanuel
Glupczynski, Youri
Rodriguez-Villalobos, Hector
De Vos, Daniel
Pirnay, Jean-Paul
Bilocq, Florence
Guery, Benoit
Tulkens, Paul M
Mingeot-Leclercq, Marie-Paule
Van Bambeke, Francoise
eng
Research Support, Non-U.S. Gov't
Pathog Dis. 2015 Oct;73(7). pii: ftv049. doi: 10.1093/femspd/ftv049. Epub 2015 Jul 22.
Abstract
Type III secretion system (T3SS) in Pseudomonas aeruginosa is associated with poor clinical outcome in acute infections. T3SS allows for injection of bacterial exotoxins (e.g. ExoU or ExoS) into the host cell, causing cytotoxicity. It also activates the cytosolic NLRC4 inflammasome, activating caspase-1, inducing cytotoxicity and release of mature IL-1beta, which impairs bacterial clearance. In addition, flagellum-mediated motility has been suggested to also modulate inflammasome response and IL-1beta release. Yet the capacity of clinical isolates to induce IL-1beta release and its relation with cytotoxicity have never been investigated. Using 20 clinical isolates from acute infections with variable T3SS expression levels and human monocytes, our aim was to correlate IL-1beta release with toxin expression, flagellar motility and cytotoxicity. ExoU-producing isolates caused massive cell death but minimal release of IL-1beta, while those expressing T3SS but not ExoU (i.e. expressing ExoS or no toxins) induced caspase-1 activation and IL-1beta release, the level of which was correlated with cytotoxicity. Both effects were prevented by a specific caspase-1 inhibitor. Flagellar motility was not correlated with cytotoxicity or IL-1beta release. No apoptosis was detected. Thus, T3SS cytotoxicity is accompanied by a modification in cytokine balance for P. aeruginosa clinical isolates that do not express ExoU.
Keywords
Bacterial Toxins/*metabolism, Cell Death/drug effects, Cell Line, Cell Survival/drug effects, Flagella/*physiology, Humans, Interleukin-1beta/*metabolism, Locomotion, Monocytes/drug effects/*microbiology/*physiology, Pseudomonas Infections/*microbiology, Pseudomonas aeruginosa/isolation & purification/*metabolism/physiology, ExoS, ExoU, TNF-alpha, flagellin, inflammasome, type three secretion system
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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