A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts.
Détails
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_AECF31D38959
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts.
Périodique
Disease models & mechanisms
ISSN
1754-8411 (Electronic)
ISSN-L
1754-8403
Statut éditorial
Publié
Date de publication
14/07/2020
Peer-reviewed
Oui
Volume
13
Numéro
7
Pages
dmm044586
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Résumé
SWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here, we present a comprehensive mouse reference spectral library (MouseRefSWATH) that permits quantification of up to 10,597 proteins (62.2% of the mouse proteome) by SWATH-MS. We exploit MouseRefSWATH to develop an analytical pipeline for species-specific deconvolution of proteomic alterations in human tumour xenografts (XenoSWATH). This method overcomes the challenge of high sequence similarity between mouse and human proteins, facilitating the study of host microenvironment-tumour interactions from 'bulk tumour' measurements. We apply the XenoSWATH pipeline to characterize an intraductal xenograft model of breast ductal carcinoma in situ and uncover complex regulation consistent with stromal reprogramming, where the modulation of cell migration pathways is not restricted to tumour cells but also operates in the mouse stroma upon progression to invasive disease. MouseRefSWATH and XenoSWATH open new opportunities for in-depth and reproducible proteomic assessment to address wide-ranging biological questions involving this important model organism.
Mots-clé
Animals, Breast Neoplasms/metabolism, Breast Neoplasms/pathology, Carcinoma, Intraductal, Noninfiltrating/metabolism, Carcinoma, Intraductal, Noninfiltrating/pathology, Cell Communication, Cell Line, Tumor, Chromatography, Liquid, Databases, Protein, Female, Heterografts, Humans, Male, Mice, Mice, Inbred C57BL, Mice, Nude, Mice, SCID, NIH 3T3 Cells, Neoplasm Proteins/metabolism, Neoplasm Transplantation, Proteome, Proteomics, Species Specificity, Stromal Cells/metabolism, Stromal Cells/pathology, Tandem Mass Spectrometry, Tumor Microenvironment, Breast cancer, DCIS, Mass spectrometry, Mouse, SWATH-MS, Xenografts
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/06/2020 20:52
Dernière modification de la notice
12/01/2022 8:12