A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts.

Details

Serval ID
serval:BIB_AECF31D38959
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts.
Journal
Disease models & mechanisms
Author(s)
Krasny L., Bland P., Burns J., Lima N.C., Harrison P.T., Pacini L., Elms M.L., Ning J., Martinez V.G., Yu Y.R., Acton S.E., Ho P.C., Calvo F., Swain A., Howard B.A., Natrajan R.C., Huang P.H.
ISSN
1754-8411 (Electronic)
ISSN-L
1754-8403
Publication state
Published
Issued date
14/07/2020
Peer-reviewed
Oui
Volume
13
Number
7
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
SWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here, we present a comprehensive mouse reference spectral library (MouseRefSWATH) that permits quantification of up to 10,597 proteins (62.2% of the mouse proteome) by SWATH-MS. We exploit MouseRefSWATH to develop an analytical pipeline for species-specific deconvolution of proteomic alterations in human tumour xenografts (XenoSWATH). This method overcomes the challenge of high sequence similarity between mouse and human proteins, facilitating the study of host microenvironment-tumour interactions from 'bulk tumour' measurements. We apply the XenoSWATH pipeline to characterize an intraductal xenograft model of breast ductal carcinoma in situ and uncover complex regulation consistent with stromal reprogramming, where the modulation of cell migration pathways is not restricted to tumour cells but also operates in the mouse stroma upon progression to invasive disease. MouseRefSWATH and XenoSWATH open new opportunities for in-depth and reproducible proteomic assessment to address wide-ranging biological questions involving this important model organism.
Keywords
Breast cancer, DCIS, Mass spectrometry, Mouse, Proteomics, SWATH-MS, Xenografts
Pubmed
Web of science
Open Access
Yes
Create date
10/06/2020 20:52
Last modification date
14/10/2020 6:23
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