The developmental proteome of Drosophila melanogaster.

Détails

ID Serval
serval:BIB_AE7A43DD17FE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The developmental proteome of Drosophila melanogaster.
Périodique
Genome research
Auteur(s)
Casas-Vila N., Bluhm A., Sayols S., Dinges N., Dejung M., Altenhein T., Kappei D., Altenhein B., Roignant J.Y., Butter F.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Statut éditorial
Publié
Date de publication
07/2017
Peer-reviewed
Oui
Volume
27
Numéro
7
Pages
1273-1285
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to explore proteome remodeling across Drosophila's life cycle, resulting in 7952 proteins, and provide a high temporal-resolved embryogenesis proteome of 5458 proteins. Our proteome data enabled us to monitor isoform-specific expression of 34 genes during development, to identify the pseudogene Cyp9f3Ψ as a protein-coding gene, and to obtain evidence of 268 small proteins. Moreover, the comparison with available transcriptomic data uncovered examples of poor correlation between mRNA and protein, underscoring the importance of proteomics to study developmental progression. Data integration of our embryogenesis proteome with tissue-specific data revealed spatial and temporal information for further functional studies of yet uncharacterized proteins. Overall, our high resolution proteomes provide a powerful resource and can be explored in detail in our interactive web interface.
Mots-clé
Animals, Drosophila Proteins/biosynthesis, Drosophila melanogaster, Gene Expression Regulation, Developmental/physiology, Proteome/biosynthesis
Pubmed
Web of science
Création de la notice
28/10/2019 13:51
Dernière modification de la notice
29/10/2019 7:26
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