The developmental proteome of Drosophila melanogaster.

Details

Serval ID
serval:BIB_AE7A43DD17FE
Type
Article: article from journal or magazin.
Collection
Publications
Title
The developmental proteome of Drosophila melanogaster.
Journal
Genome research
Author(s)
Casas-Vila N., Bluhm A., Sayols S., Dinges N., Dejung M., Altenhein T., Kappei D., Altenhein B., Roignant J.Y., Butter F.
ISSN
1549-5469 (Electronic)
ISSN-L
1088-9051
Publication state
Published
Issued date
07/2017
Peer-reviewed
Oui
Volume
27
Number
7
Pages
1273-1285
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we apply label-free quantitative proteomics to explore proteome remodeling across Drosophila's life cycle, resulting in 7952 proteins, and provide a high temporal-resolved embryogenesis proteome of 5458 proteins. Our proteome data enabled us to monitor isoform-specific expression of 34 genes during development, to identify the pseudogene Cyp9f3Ψ as a protein-coding gene, and to obtain evidence of 268 small proteins. Moreover, the comparison with available transcriptomic data uncovered examples of poor correlation between mRNA and protein, underscoring the importance of proteomics to study developmental progression. Data integration of our embryogenesis proteome with tissue-specific data revealed spatial and temporal information for further functional studies of yet uncharacterized proteins. Overall, our high resolution proteomes provide a powerful resource and can be explored in detail in our interactive web interface.
Keywords
Animals, Drosophila Proteins/biosynthesis, Drosophila melanogaster, Gene Expression Regulation, Developmental/physiology, Proteome/biosynthesis
Pubmed
Web of science
Create date
28/10/2019 12:51
Last modification date
29/10/2019 6:26
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