Typing of Listeria monocytogenes by monocin and phage receptors

Détails

ID Serval
serval:BIB_ADBB856B5803
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Typing of Listeria monocytogenes by monocin and phage receptors
Périodique
International Journal of Food Microbiology
Auteur(s)
Bannerman  E., Boerlin  P., Bille  J.
ISSN
0168-1605
Statut éditorial
Publié
Date de publication
08/1996
Peer-reviewed
Oui
Volume
31
Numéro
1-3
Pages
245-62
Notes
Comparative Study
Journal Article --- Old month value: Aug
Résumé
One hundred strains of Listeria monocytogenes from both sporadic and epidemic cases were typed by monocin production combined with phage receptor and reverse phage receptor methods. The monocin-phage combination gave 72 types with 100% typability and 97% reproducibility. The results were compared to those of serotyping, phage typing, ribotyping, multilocus enzyme electrophoresis, restriction enzyme analysis and RAPD (random amplification of polymorphic DNA). The monocin/phage types were comparable in terms of discrimination with other methods for epidemiological investigations. The index of discrimination of using the monocin typing and phage receptor/reverse phage receptor method combination (0.99) for both the 87 epidemiologically unrelated strains and the epidemiologically important serogroup 4 strains was the highest of the seven different methods analysed. This combination of methods was simple, highly discriminatory and reproducible and can be carried out in a non-specialized laboratory. However, like most of the other Listeria typing methods, both the method and the indicator test strains need to be standardized.
Mots-clé
*Bacterial Typing Techniques Bacteriocins/*classification Bacteriophage Typing Listeria monocytogenes/*classification Receptors, Virus/*analysis Reproducibility of Results
Pubmed
Web of science
Création de la notice
11/02/2008 13:39
Dernière modification de la notice
20/08/2019 16:17
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